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Repository Storage Core

The newly expanded JHBR Repository Core is a state-of-the-art cryogenic storage facility located on Shannon Drive in Baltimore, Maryland (3 miles from JHSPH).  Biological specimens are stored in high capacity vapor phase liquid nitrogen cryogenic storage tanks. Currently, 60 cryogenic units, by Taylor Wharton, are maintained with 24-hour, 365-day security and daily temperature monitoring. The capacity of these units ranges from 20,000, 80,000 and 94,000 vials (2 inch, 10 X 10 freezer boxes).  Cryogenic units not only provide ample storage and temperature security, but have also helped JHBR meet and exceed JHSPH’s goals for energy efficiency.

Data Management:

JHBR uses Freezerworks (FW), state-of-the-art sample cataloging software. Each aliquot created in the JHBR laboratories is labeled with a FW label. This label includes information such as sample ID, sample date, sample type and can endure temperatures colder than -190. Every label contains a bar code that is used to scan the aliquot into FW, which allows every aliquot to be tracked and linked to the sample record.

Sample Handling and Retrieval:

All specimens trusted to JHBR laboratories and repositories are handled with the same standard of excellence.  JHBR strives to maintain sample integrity through proper technique and highly trained staff utilizing the latest in cryogenic and automated platforms. Once specimens are frozen, they are transferred from working freezers into the cryogenic repository at a constant -80 degrees C to avoid degradation in sample quality due to temperature fluctuations. The same is true when specimens are requested for retrieval.

In conjunction with JHBR's short and long-term storage, specimen retrieval and shipping is a very important service. This may involve pulling and shipping specimens or aliquotting daughter tubes.  Following strict standard operating procedures, JHBR ensures the protection of sample integrity and identification for each tube.  Specimens can be shipped to local, national or international locations…anywhere in the world on either dry ice or liquid nitrogen for cryopreserved cells. JHBR lab staff is fully IATA certified to meet national and international regulations for biologic specimen transfer.

Related Links:

AIRGASTaylor Wharton Cryoscience

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