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Research

Testing Core

JHBR Testing Core laboratory, located on the 6th floor of Johns Hopkins School of Public Health, offers diagnostic serologic and virologic testing. Since its inception in 2002, JHBR Testing Core has achieved and maintained the highest level of international standards (CAP, VQA, AAB) for test result accuracy and quality assurance compliance. In July of 2010, JHBR Testing Core received certification through the Clinical Laboratory Improvement Amendment (CLIA) in Immunology and Molecular Biology, allowing JHBR to process and test specimens for research and clinical purposes.

Methods/Equipment:

Virology/HIV-1 RNA:

JHBR utilizes the most up-to-date automated platform for real time PCR and viral load quantification.  The COBAS Ampliprep and COBAS TaqMan by ROCHE are fully automated for sample preparation, amplification (real-time PCR) and quantification (FRET analysis).

Immunology/Enzyme Immunoassay (EIA):

JHBR has relied on the EVOLIS as a self-contained microplate processor for automated EIA testing. Manufactured by Bio-Rad for fifteen years, the EVOLIS contains a pipettor, incubator, washer, and calorimeter for enzyme immunoassays. EVOLIS streamlines workflow for moderate to high work load volumes and optimizes productivity, using a scheduler program to simplify instrument operation. The current serological markers of interest include: HIV-1/2 Antibodies, HIV-1 Antigen, HBsAg, HBsAb, HBcAb and HCV, although the system is capable of detecting a number of different markers.

In addition to the fully automated serological tests performed on the EVOLIS, JHBR offers The OraQuick ADVANCE® Rapid HIV-1/2 AntiBody Test as well as the BioRad Geenius™ HIV 1/2 Supplemental Assay for confirmation testing of individual antibodies of Human Immunodeficiency Virus Types 1 and 2 (HIV-1 and HIV-2).