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Mark J. Kohr, PhD

  • Assistant Professor

Departmental Affiliations

Contact Information

615 N. Wolfe Street
Room E7616
Baltimore, Maryland 21205

443-287-2721
410-955-0617

Laboratory Website
LinkedIn
Research Gate

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Education

PhD, The Ohio State University, 2009

Overview

Ischemic heart disease is a leading cause of death in both men and women in the United States. Research in the Kohr Laboratory of Cardiovascular Redox Signaling seeks to identify novel, redox-dependent biological mechanisms that reduce ischemic injury in the heart. Current projects center on the protective role of myocardial protein S-nitrosation, with a major focus on sex-specific differences. The female heart is of particular interest, since it is naturally protected from ischemic injury. To investigate these protective mechanisms, we have developed a number of cutting-edge mass spectrometry-based proteomic methodologies to map sites of S-nitrosation and other redox-based modifications, and we use these in tandem with cellular, molecular, and biochemical methods to assess the effects of S-nitrosation on the function, stability, and localization of specific protein targets. We also utilize a number of physiological approaches to evaluate myocardial function, including the Langendorff-perfused heart preparation, in vivo pressure-volume loop analysis, and echocardiography. Additional projects seek to evaluate the impact of environmental toxicants (i.e., arsenic) on myocardial physiology and cardioprotective signaling. By defining female-specific and other protective signaling pathways in the heart, and assessing the potential environmental impact on these pathways, we seek to identify key therapeutic targets for the treatment of ischemic heart disease in both men and women.

Honors and Awards

Research Project Grant (R01), National Heart, Lung and Blood Institute (2017-2022)

Seed Grant, Foundation for Gender Specific Medicine (2017-2018)

Teaching Excellence Award (Terms 3 and 4), Johns Hopkins Bloomberg School of Public Health, Baltimore, MD (2017)

Mamie Doud Eisenhower Memorial Award, 69th Annual Affair of the Heart, The Women’s Board of the American Heart Association (2017)

Fellow of the American Heart Association, Basic Cardiovascular Sciences Research Council (2016)

Grant-in-Aid Award, American Heart Association (2016-2018)

Early Career Poster Award, Arteriosclerosis, Thrombosis & Vascular Biology Research Council, American Heart Association Annual Scientific Sessions (2016)

Young Investigator Award Competition Finalist (Senior Category), International Society for Heart Research North American Section Meeting (2014)

Early Career Investigator Event Vice Chair, International Society for Heart Research North American Section Meeting (2014)

Pathway to Independence Award (K99/R00), National Heart, Lung and Blood Institute (2013-2018)

Early Career Investigator Travel Award, XXI World Congress of the International Society for Heart Research (2013)

Beginning Grant-in-Aid Award, American Heart Association (2012-2014)

Gordon Research Seminar Chair, Cardiac Regulatory Mechanisms Gordon Research Conference (2012)

International Poster Presentation Award, International Society for Heart Research North American Section Meeting (2012)

Young Investigator Award Competition Winner (Junior Category), International Society for Heart Research North American Section Meeting (2011)

Best of the American Heart Association’s Specialty Conferences (Abstract), Basic Cardiovascular Sciences Scientific Sessions (2011)

Basic Cardiovascular Sciences New Investigator Travel Award, Basic Cardiovascular Sciences Scientific Sessions (2011)

Fellows Award for Research Excellence, National Institutes of Health (2011)

Excellence in Basic Research Award, Johns Hopkins University School of Medicine Department of Pathology (2011)

Early Career Investigator Travel Award, XX World Congress of the International Society for Heart Research (2010)

National Research Service Award, National Heart Lung and Blood Institute (2009-2012)

Travel Award, The Ohio State University Department of Physiology and Cell Biology (2009)

D.M. Davis Heart and Lung Research Institute Poster Presentation Competition Winner, The Ohio State University (2008)

Travel Award, NIH National Graduate Student Research Conference (2008)

D.M. Davis Heart and Lung Research Institute Oral Presentation Competition Winner, The Ohio State University (2008)

Predoctoral Fellowship Award, American Heart Association (2007-2009)

E.F. Hayes Graduate Research Forum Finalist, The Ohio State University (2007)

D.M. Davis Heart and Lung Research Institute Travel Award, The Ohio State University (2005)

B.A. awarded with honors, St. Mary’s College of Maryland (2002)

Outstanding Oral Presentation of a St. Mary’s Project in Biology, St. Mary’s College of Maryland (2002)

Phi Kappa Phi Honor Society (2006-Present)

Tri-Beta Biological Honor Society (1998-2002)

  • Cardiovascular Disease
  • Cardiovascular Physiology
  • Sex Differences
  • Redox Biology
  • Cellular Biology
  • Molecular Biology
  • Cellular Signaling
  • Protein Oxidation
  • Mass Spectrometry & Proteomics
  • Electrophysiology
  • Fluorescence Microscopy

Select publications (from 34 total publications; *Authors contributed equally, #Corresponding author, ^Post-doctoral fellow, &Graduate student, %Undergraduate student):

  • Kohr, M.J., Sun, J., Aponte, A., Wang, G., Gucek, M., Murphy, E., Steenbergen, C. Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture. Circ Res. 108:418-426; 2011. Cited by Faculty of 1000 as a Recommended Article
  • Kohr, M.J., Aponte, A., Sun, J., Gucek, M., Steenbergen, C., Murphy, E., Measurement of S-nitrosylation occupancy in the myocardium with cysteine-reactive tandem mass tags. Circ Res.111:1308-12; 2012. Circulation Research Editor’s Pick, Comment in: Murray, C.I., Van Eyk, J.E. A twist on quantification: measuring the site occupancy of S-nitrosylation. Circ Res. 111:1253-5; 2012.
  • Kohr, M.J.#,*, Evangelista, A.*, Ferlito, M., Steenbergen, C., Murphy, E. S-nitrosylation of TRIM72 at cysteine 144 is critical for protection against oxidation-induced protein degradation and cell death. J Mol Cell Cardiol. 69:67-74; 2014. Comment in: X’avia Chan, C.Y., Wang, D., Cadeiras, M., Deng, M.C., Ping, P. S-nitrosylation of TRIM72 mends the broken heart: A molecular modifier-mediated cardioprotection. J Mol Cell Cardiol. 72:292-295; 2014.
  • Shao, Q.,^ Fallica, J.,^ Casin, K.,& Murphy, E., Steenbergen, C., Kohr, M.J.# Characterization of the sex-dependent myocardial S-nitrosothiol proteome. Am J Physiol Heart Circ Physiol. 310(4): H505-15; 2016.
  • Shao,Q.,^ Casin, K.M.,& Mackowski, N., Murphy, E., Steenbergen, C., Kohr, M.J.# Adenosine A1 receptor activation increases myocardial protein S-nitrosothiols and elicits protection in male and female hearts. PLoS One. 12(5): e0177315; 2017.