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Course Catalog

260.613.01 Techniques in Molecular Biology

Department:
Molecular Microbiology and Immunology
Term:
3rd term
Credits:
3 credits
Academic Year:
2019 - 2020
Location:
East Baltimore
Dates:
Mon 01/06/2020 - Fri 01/10/2020
Class Times:
  • M Tu W Th F,  9:00am - 5:00pm
Lab Times:
  • M Tu W Th F,  9:00am - 5:00pm
Auditors Allowed:
No
Grading Restriction:
Letter Grade or Pass/Fail
Contact:
Richard Markham
Course Instructor :
Resources:
Prerequisite:

None

Description:

Practical knowledge of molecular biology techniques is an essential part of any biological science graduate program.

Supplements the scientific classroom experience of students in biological science graduate programs by providing hands-on experience with essential core molecular biology techniques. Involves five days of intensive hands-on laboratory instruction along with the science behind the applications. The methodologies focused on are the following: polymerase chain reaction (PCR) DNA amplification, quantitative PCR, DNA and protein gel chromatography, Western blotting, transformation of bacteria, and expression of heterologous proteins by bacteria.

Learning Objectives:

Upon successfully completing this course, students will be able to:

  1. Explain how these methodologies work scientifically and in what context they should be employed to answer scientific questions.
  2. Employ laboratory laboratory techniques that are widely used in the areas of immunology and infectious disease research.
  3. Compare and contrast different methodologies in order to solve biological problems.
Methods of Assessment:

This course is evaluated as follows:

  • 50% Final Exam
  • 50% Lab notebook quality

Enrollment Restriction:

Masters students interested in learning lab techniques

Instructor Consent:

Consent required for some students

Consent Note:

Consent required for undergraduate students.

For consent, contact:

rmarkha1@jhu.edu

Special Comments:

The laboratory portion is offered on a schedule of 6-8 hours/day during the first week of the Winter intersession.