120.861.01 Special Topics in Biochemistry: X-Ray Crystallography-A Hands On Workshop
- Biochemistry and Molecular Biology
- Summer term
- 3 credits
- Academic Year:
- 2019 - 2020
- East Baltimore
Some basic knowledge of UNIX or Linux is desirable.
Enables students to carry out all key steps to successfully solve and refine a protein crystal structure. Theoretical aspects are followed by application to various problem sets. Topics include tricks for data collection, data processing and collection. Touches upon all standard techniques such as molecular replacement, SAD phasing and MAD phasing, both in theory and then applied in practical context with previously collected data. Identification of unknown ligand densities and model refinement lead to the last part of preparing publication quality figures using PyMol.
- Learning Objectives:
- Process and analyze x-ray data from protein crystals.
- Methods of Assessment:
Students are evaluated on their participation in classroom discussions and also on completion of assigned homework and tutorial data.
- Enrollment Restriction:
- Instructor Consent:
Consent required for all students
- Consent Note:
Permission of instructor required.
- For consent, contact:
- Special Comments:
Students can visit http://lupo/jhsph.edu/boschlab/Xray_Workshop.html for updated information about the course.