615 N. Wolfe Street, Room E7032
Baltimore , Maryland 21205
(410) 955 4235
(410) 955 0617
Research interests in my laboratory center around the development, validation and application of analytical methods to biomonitor human exposure to carcinogens. The range of biomarkers studied includes carcinogen metabolites in urine, protein and DNA carcinogen adducts in plasma or serum and carcinogen-induced somatic mutations in free-circulating DNA in plasma or serum. Analytical techniques include Gas Chromatography/Negative Chemical Ionization Mass spectrometry and High Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry.
Our laboratory is currently developing a quantitative technique called Short Oligonucleotide Mass Analysis (SOMA), which can be used for the analysis of sequence variants (somatic mutations, single nucleotide polymorphisms) in free-circulating DNA in plasma and serum. The technique has been applied to detect serum levels of TP53 mutations caused by exposure to Aflatoxin B1.
Mass Spectrometry, Short Oligonucleotide Mass Analysis, SOMA, Biomarkers of carcinogen exposure
Khaw K-T, Friesen MD, Riboli E, Luben R, Wareham N. Plasma Phospholipid Fatty Acid Concentration and Incident Coronary Heart Disease in Men and Women: The EPIC-Norfolk Prospective Study. PLoS Med. 2012; 9: e1001255. [Epub ahead of print]
Weinstein SJ, Peters U, Ahn J, Friesen MD, Riboli E, Hayes, RB, & Albanes D. Serum α-Tocopherol and γ-Tocopherol Concentrations and Prostate Cancer Risk in the PLCO Screening Trial: A Nested Case-Control Study. PLoS ONE. 2012; 7:e40204. [Epub ahead of print]
Villar S, Ortiz-Cuaran S, Abedi-Ardekani B, Gouas D, Nogueira da Costa A, Plymoth A, Khuhaprema T, Kalalak A, Sangrajrang S, Friesen MD, Groopman JD and Hainaut P. Aflatoxin-Induced TP53 R249S Mutation in HepatoCellular Carcinoma in Thailand: Association with Tumors Developing in the Absence of Liver Cirrhosis. PLoS ONE. 2012; 7: e37707 [Epub ahead of print]
Gouas DA, Villar S, Ortiz-Cuaran S, Legros P, Ferro G, Kirk GD, Lesi OA, Mendy M, Bah E, Friesen MD, Groopman J, Chemin I, Hainaut P. TP53 R249S mutation, genetic variations in HBX and risk of hepatocellular carcinoma in The Gambia. Carcinogenesis. 2012; 33:1219-24.
Rennie A, Luo L, Jarow J, Wright WW, Brown TR, Chen H, Zirkin BR, and Friesen MD. Simultaneous Quantification of Steroids in Rat Intratesticular Fluid by HPLC Isotope Dilution-Tandem Mass Spectrometry. J Androl. 2012; 33:691-98.