Skip Navigation

Faculty Directory

Mark J. Kohr, PhD

  • Assistant Professor

Departmental Affiliations

Contact Information

615 N. Wolfe Street
Room E7616
Baltimore, Maryland 21205

443-287-2721
410-955-0617

Laboratory Website
LinkedIn
Research Gate

View Current Courses

Education

PhD, The Ohio State University, 2009

Overview

Nitroso-redox disequilibrium is a common hallmark of many forms of cardiovascular disease, which is the leading cause of death in the United States. My research activities seek to define the underlying redox-sensitive signaling pathways and the mechanistic consequences of redox-based post-translational protein modifications in healthy and diseased myocardium. S-nitrosylation, which results from the addition of a nitric oxide moiety to a cysteine thiol, is one example of a redox-based modification that has been implicated as a critical regulator of many processes that govern normal cellular physiology, including protein function and stability, protein-protein interaction, and protein localization.

To examine these modifications, my laboratory has developed several novel proteomic approaches to map specific cysteine modification sites and assess occupancy levels in the heart using mass spectrometry. My laboratory also utilizes additional cutting-edge methodologies, including site-directed mutagenesis and gene editing strategies, to further investigate the role of S-nitrosylation and other redox-based modifications in regulating protein function, stability and localization. Additional research efforts center on sex differences in S-nitrosothiol signaling, as well as elucidating the dynamics of protein S-nitrosylation including mechanisms for the addition and removal of the modification, target specificity, spatial localization, mechanistic consequences, and interplay with other redox-based modifications. A better understanding of protein S-nitrosylation and other redox-based modifications is critical for the development of more effective therapeutic strategies to effectively combat cardiovascular disease.

Honors and Awards

Grant-in-Aid Award, American Heart Association (2016-2018)

Young Investigator Award Competition Finalist (Senior Category), International Society for Heart Research North American Section Meeting (2014)

Early Career Investigator Event Vice Chair, International Society for Heart Research North American Section Meeting (2014)

Pathway to Independence Award (K99/R00), National Heart Lung and Blood Institute (2013-2018)

Early Career Investigator Travel Award, XXI World Congress of the International Society for Heart Research (2013)

Beginning Grant-in-Aid Award, American Heart Association (2012-2014)

Gordon Research Seminar Chair, Cardiac Regulatory Mechanisms Gordon Research Conference (2012)

International Poster Presentation Award, International Society for Heart Research North American Section Meeting (2012)

Young Investigator Award Competition Winner (Junior Category), International Society for Heart Research North American Section Meeting (2011)

Best of the American Heart Association’s Specialty Conferences (Abstract), Basic Cardiovascular Sciences Scientific Sessions (2011)

Basic Cardiovascular Sciences New Investigator Travel Award, Basic Cardiovascular Sciences Scientific Sessions (2011)

Fellows Award for Research Excellence, National Institutes of Health (2011)

Excellence in Basic Research Award, Johns Hopkins University School of Medicine Department of Pathology (2011)

Early Career Investigator Travel Award, XX World Congress of the International Society for Heart Research (2010)

National Research Service Award, National Heart Lung and Blood Institute (2009-2012)

Travel Award, The Ohio State University Department of Physiology and Cell Biology (2009)

D.M. Davis Heart and Lung Research Institute Poster Presentation Competition Winner, The Ohio State University (2008)

Travel Award, NIH National Graduate Student Research Conference (2008)

D.M. Davis Heart and Lung Research Institute Oral Presentation Competition Winner, The Ohio State University (2008)

Predoctoral Fellowship Award, American Heart Association (2007-2009)

E.F. Hayes Graduate Research Forum Finalist, The Ohio State University (2007)

D.M. Davis Heart and Lung Research Institute Travel Award, The Ohio State University (2005)

B.A. awarded with honors, St. Mary’s College of Maryland (2002)

Outstanding Oral Presentation of a St. Mary’s Project in Biology, St. Mary’s College of Maryland (2002)

Phi Kappa Phi Honor Society (2006-Present)

Tri-Beta Biological Honor Society (1998-2002)

  • Cardiovascular disease
  •   Cardiac physiology
  •   Electrophysiology
  •   Proteomics
  •   Reactive nitrogen species
  •   Nitric oxide
  •   S-nitrosylation
  •   Reactive oxygen species
  •   Nitroso-redox balance
  •   Oxidative stress
  • Sex differences

Select publications (from 32 total publications):

  • Kohr, M.J., Sun, J., Aponte, A., Wang, G., Gucek, M., Murphy, E., Steenbergen, C. Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture. Circ Res. 108:418-426; 2011. Cited by Faculty of 1000 as a Recommended Article
  • Kohr, M.J., Aponte, A., Sun, J., Gucek, M., Steenbergen, C., Murphy, E., Measurement of S-nitrosylation occupancy in the myocardium with cysteine-reactive tandem mass tags. Circ Res.111:1308-12; 2012. Circulation Research Editor’s Pick, Comment in: Murray, C.I., Van Eyk, J.E. A twist on quantification: measuring the site occupancy of S-nitrosylation. Circ Res. 111:1253-5; 2012.
  • Kohr, M.J., Murphy, E., Steenbergen, C. GAPDH acts as a mitochondrial trans-S-nitrosylase in the myocardium. PLOS One. 9:e111448; 2014.
  • Kohr, M.J., Evangelista, A., Ferlito, M., Steenbergen, C., Murphy, E. S-nitrosylation of TRIM72 at cysteine 144 is critical for protection against oxidation-induced protein degradation and cell death. J Mol Cell Cardiol. 69:67-74; 2014. Comment in: X’avia Chan, C.Y., Wang, D., Cadeiras, M., Deng, M.C., Ping, P. S-nitrosylation of TRIM72 mends the broken heart: A molecular modifier-mediated cardioprotection. J Mol Cell Cardiol. 72:292-295; 2014.
  • Shao, Q., Fallica, J., Casin, K.,& Murphy, E., Steenbergen, C., Kohr, M.J. Characterization of the sex-dependent myocardial S-nitrosothiol proteome. Am J Physiol Heart Circ Physiol. 310(4): H505-15; 2016.
  • S-nitrosylation and Cardioprotection