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120.613.01 NUCLEIC ACID CHEMISTRY

Term: 3rd term
Credits: 3 credits
Contact: Paul Miller
Academic Year: 2013 - 2014
Course Instructors:
Description:

Discusses nucleic acid structure, and also describes techniques for manipulating and analyzing nucleic acids, including gel electrophoresis, PCR, and DNA sequencing. Reviews methods used to synthesize nucleosides, nucleotides and oligonucleotides, and chemical reactions that lead to modifications of nucleic acids. Additional topics include: nucleic acid molecular beacons and molecular wires; antisense and antigene oligonucleotides; nucleic acid nanostructures and "machines", and nucleic acid aptamers.

Learning Objective(s):
This course will prepare you to be able to do the following:
Design schemes to synthesize nucleosides and oligonucleotides
Predict the products of reactions between nucleic acids and various chemical agents, ionizing and ultraviolet radiation
Understand the design and function of antisense, antigene and siRNAs
Understand the design and synthesis of nucleic acid nanostructures and machines
Understand how nucleic acid aptamers are applied to biological problems

Methods of Assessment: Final exam at end of term.
Location: East Baltimore
Class Times:
  • Monday 3:30 - 4:50
  • Wednesday 3:30 - 4:50
Enrollment Minimum: 4
Enrollment Restriction: Undergraduate students are prohibited from registering for this course.
Instructor Consent: Consent required for all students

Student should contact instructor for consent to take course.

For consent, contact: pmiller@jhsph.edu
Prerequisite:

Organic Chemistry and Biochemistry and/or Molecular Biology

Auditors Allowed: Yes, with instructor consent
Grading Restriction: Letter Grade or Pass/Fail